Alix Antibody (3A9)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65678
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Bovine
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3A9
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Alix-GST fusion protein
Reactivity Notes
Predicted cross-reactivities: Rat, Xenopus, Mouse, Human
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Alix Antibody (3A9)
Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]
Western Blot: Alix Antibody (3A9) [NB100-65678] - B. Western blot for the exosomal marker Alix and Nef in cells and exosomes (exNef). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31344124) licensed under a CC-BY license.Western Blot: Alix Antibody (3A9)BSA Free [NB100-65678]
Western Blot: Alix Antibody (3A9) [NB100-65678] - Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (STAR13B)Western Blot: Alix Antibody (3A9) [NB100-65678] -
Western Blot: Alix Antibody (3A9) [NB100-65678] - Nef-containing exosomes deliver Nef to macrophages.A—Size distribution of the extracellular vesicles secreted by HEK293 cells determined by EM; Inset–EM micrograph of the vesicles; bar– 200 nm. B—Western blot for the exosomal marker Alix & Nef in cells & exosomes (exNef); C—Western blot for the indicated amounts of rNef & in a typical preparation of exNef (10 μg of exosomal protein); D, E–Time-course of exosome uptake quantitated by confocal microscopy; F–Time-course of exosome uptake quantitated by fluorimetry; percentage of added exosomes that was taken up is shown; G–Cells were incubated with exosomes for 48 h, excess exosomes was washed out & cells incubated for the indicated periods of time in exosome-free medum; retained fluorscence of the exosome stain PKH67 was assessed using confocal microscopy; H–Visualisation of Nef-GFP inside the cells after exposure to exNef-GFP (5 μg/ml of exosomal protein) after staining with anti-GFP antibody. Scale bars– 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31344124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Alix Antibody (3A9)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34298673). For IHC - This product does not require protein digestion pre-treatment of paraffin sections.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alix
Alternate Names
AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein
Gene Symbol
PDCD6IP
UniProt
Additional Alix Products
Product Documents for Alix Antibody (3A9)
Product Specific Notices for Alix Antibody (3A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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