Alix Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25272

Novus Biologicals, part of Bio-Techne
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NBP3-25272
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alix Antibodies »

Product Summary for Alix Antibody

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Alix using the following amino acid sequence: GVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alix Antibody

Alix Antibody Immunohistochemistry-Paraffin: Alix Antibody [NBP3-25272]

Immunohistochemistry-Paraffin: Alix Antibody [NBP3-25272]

Staining of human small intestine shows cytoplasmic positivity in glandular cells.
Alix Antibody Western Blot: Alix Antibody [NBP3-25272]

Western Blot: Alix Antibody [NBP3-25272]

Analysis in human cell line RT-4 and human cell line U-251 MG.

Applications for Alix Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alix

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Alternate Names

AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein

Gene Symbol

PDCD6IP

Additional Alix Products

Product Documents for Alix Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alix Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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