ALK-1 Antibody (124)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89257

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 124

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ALK-1 Antibodies »

Product Summary for ALK-1 Antibody (124)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ALK-1 (Accession#: NP_000011.2; Met1-Gln118).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ALK-1 Antibody (124)

Western Blot: ALK-1 Antibody (124) [NBP2-89257]

Western Blot: ALK-1 Antibody (124) [NBP2-89257]

Western Blot: ALK-1 Antibody (124) [NBP2-89257] - Lane A: MCF7 Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:41 kDa
Immunoprecipitation: ALK-1 Antibody (124) [NBP2-89257]

Immunoprecipitation: ALK-1 Antibody (124) [NBP2-89257]

Immunoprecipitation: ALK-1 Antibody (124) [NBP2-89257] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate2 uL anti-ALK1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-ALK1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size: 56 kDa

Applications for ALK-1 Antibody (124)

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALK-1

LTK, ALK and Ros have been identified as receptor tyrosine kinases having sequence similarity to the insulin receptor subfamily of kinases. LTK (leukocyte tyrosine kinase) is expressed in murine B lymphocyte precursors and has also been found in forebrain neurons. ALK (anaplastic lymphoma kinase) is normally highly expressed, specifically in the nervous system. A truncated form containing the catalytic domian of ALK is expressed as the result of a translocation occurring in many non-Hodgkin's lymphomas. The c-Ros gene was originally identified in mutant form as an oncogene. Ros is normally expressed in a small number of epithelial cell types and may play a role in epithelial development.

Long Name

Activin Receptor-like Kinase 1

Alternate Names

ACVRL1, ALK1

Gene Symbol

ACVRL1

Additional ALK-1 Products

Product Documents for ALK-1 Antibody (124)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALK-1 Antibody (124)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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