Key Product Details

Species Reactivity

Human, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ALPL/597

Concentration

0.2 mg/ml

View all Alkaline Phosphatase, Tissue Non-Specific Antibodies »

Product Specifications

Immunogen

Recombinant human Alkaline Phosphatase, Tissue Non-Specific protein (Uniprot: P05186)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54376)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597)

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).
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Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

UniProt

P05186

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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