Alkaline Phosphatase, Tissue Non-Specific Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32948

Novus Biologicals, part of Bio-Techne
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NBP1-32948
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Human

Predicted:

Feline (90%), Rat (91%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alkaline Phosphatase, Tissue Non-Specific Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase, Tissue Non-Specific. The exact sequence is proprietary.

Reactivity Notes

Cat (90%), Bovine (89%), Dog (89%).

Localization

Cell membrane, Lipid-anchor, GPI-anchor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody

Alkaline Phosphatase, Tissue Non-Specific Antibody

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase, Tissue Non-Specific antibody (NBP1-32948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948]

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948]

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Deprivation of NO induces bi-lineage phenotype and expression of stem cell markers in mammary epithelial cells both in vivo and in 3D cultures. Representative images of drug-treated mammary glands stained for stem cell markers: alkaline phosphatase (AP) (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-43239-x) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.

Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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