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Allophycocyanin Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8927R

R&D Systems, part of Bio-Techne
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FAB8927R-100UG

Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 936809

Product Specifications

Immunogen

Allophycocyanin isolated from red algae
Accession # P25054

Specificity

Detects Allophycocyanin in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Allophycocyanin Alexa Fluor® 647-conjugated Antibody

Detection of Allophycocyanin (APC) antibody in Human PBMCs stained with APC-Cy7-Conjugated Anti-Human CD4 antibody by Flow Cytometry. 

Detection of Allophycocyanin (APC) in Human PBMCs stained with APC-Cy7-Conjugated Anti-Human CD4 by Flow Cytometry. 

Human peripheral blood mononuclear cell (PBMCs) stained with APC-Cy7-conjugated Mouse Anti-Human CD4 Monoclonal Antibody were stained with (A) Mouse Anti-Allophycocyanin Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8927R) or (B) isotype control antibody (IC0041R). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Allophycocyanin Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) stained with APC-Cy7-conjugated Mouse Anti-Human CD4 Monoclonal Antibody
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Allophycocyanin

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Alternate Names

APC

UniProt

Additional Allophycocyanin Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Allophycocyanin Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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