alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32028
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A4-G2-A3-R
Concentration
1 mg/ml
Product Summary for alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
Immunogen
Recombinant protein within Human alpha 1-Acid Glycoprotein aa 1-201 / 201. (Uniprot: P02763)
Localization
Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
Western Blot: alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R) [NBP3-32028]
Western Blot: alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R) [NBP3-32028] - Western blot analysis of alpha 1-Acid Glycoprotein on different lysates with Mouse anti-alpha 1-Acid Glycoprotein antibody (NBP3-32028) at 1/1,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: HCT 116 cell lysate (20 ug/Lane)
Lane 4: 293T cell lysate (20 ug/Lane)
Lane 5: Human liver tissue lysate (40 ug/Lane)
Predicted band size: 24 kDa
Observed band size: 37/45 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32028) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha 1-Acid Glycoprotein
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Gene Symbol
ORM1
Additional alpha 1-Acid Glycoprotein Products
Product Documents for alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
Product Specific Notices for alpha 1-Acid Glycoprotein Antibody (A4-G2-A3-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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