alpha 2-Macroglobulin Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-939

Novus Biologicals, part of Bio-Techne
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NB600-939-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

View all alpha 2-Macroglobulin Antibodies »

Product Specifications

Immunogen

alpha 2-Macroglobulin [Human Plasma] (Uniprot: P01023)

Reactivity Notes

Cross reactivity against alpha 2-Macroglobulin from other sources is unknown.

Localization

Secreted

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified alpha-2-MACROGLOBULIN [Human Plasma]

Scientific Data Images for alpha 2-Macroglobulin Antibody [Biotin]

Western Blot: alpha 2-Macroglobulin Antibody [Biotin] [NB600-939] - Lane 1: alpha 2-Macroglobulin Antibody. Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
Western Blot: alpha 2-Macroglobulin Antibody [Biotin] [NB600-939] - Goat anti Alpha-2-Macroglobulin antibody was used to detect alpha 2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1:10K in TBS 1 hr RT.
alpha 2-Macroglobulin Antibody [Biotin]

alpha 2-Macroglobulin Antibody [Biotin]

Goat anti Alpha-2-Macroglobulin antibody ( lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 649 conjugated Donkey anti goat ( lot 20834 1:10K in TBS/) 1 hr RT. Images were collected using the BioRad VersaDoc System.

Applications for alpha 2-Macroglobulin Antibody [Biotin]

Application
Recommended Usage

ELISA

1:20000-1:100000

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of alpha-2-MACROGLOBULIN in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: alpha 2-Macroglobulin

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Gene Symbol

A2M

UniProt

P01023 (Human)

Additional alpha 2-Macroglobulin Products

Product Documents for alpha 2-Macroglobulin Antibody [Biotin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha 2-Macroglobulin Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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