alpha-2A Adrenergic R/ADRA2A Antibody

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452]

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-2A Adrenergic R/ADRA2A protein expression. Nitrergic neuron alpha2-,F(5, 12)=16.50,p<.0001 protein profiles are depicted in Panels 3B; GABAergic neuron alpha2-,F(5, 12)=10.47,p<.0001 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452]

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody.

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] -

Western Blot: alpha-2A Adrenergic R/ADRA2A Antibody [NBP2-22452] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha1-), alpha2- ( alpha2-), or beta1- ( beta1-) AR protein expression. Nitrergic neuron alpha1-, F(5, 12) = 10.51, p = .0005; alpha2-, F(5, 12) = 16.50, p < .0001; & beta1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha1-, F(5, 12) = 5.52, p = .007; alpha2-, F(5, 12) = 10.47, p < .0001; & beta1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha1-AR = alpha1 adrenergic receptor; alpha2-AR = alpha2 adrenergic receptor; beta1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.