alpha-Actinin 1 Antibody (A1G2-R)

Western Blot: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030]

Western Blot: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030] - Western blot analysis of alpha-Actinin 1 on different lysates with Mouse anti-alpha-Actinin 1 antibody (NBP3-32030) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 103 kDa
Observed band size: 103 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32030) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030]

Immunohistochemistry: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha-Actinin 1 antibody (NBP3-32030) at 1/6,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32030) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.