Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI7A4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all alpha Actinin 1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

102.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free

Western Blot: alpha-Actinin 1 Antibody (OTI7A4)Azide and BSA Free [NBP2-70078]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4)Azide and BSA Free [NBP2-70078]

Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078]

Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078]

Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078]

Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078]

Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
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Applications for alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha Actinin 1

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Long Name

Actinin alpha 1

Alternate Names

ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15

Gene Symbol

ACTN1

Additional alpha Actinin 1 Products

Product Documents for alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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