alpha COP I Antibody (3W6Y6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15839

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15839-100ul
NBP3-15839-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3W6Y6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha COP I Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human alpha COP I (P53621). MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGGDDYKIKVWNYKLRRCLFTLLGHLDYIRTTF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha COP I Antibody (3W6Y6)

Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839] - Western blot analysis of extracts of various cell lines, using alpha COP I Rabbit mAb (NBP3-15839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Immunohistochemistry-Paraffin: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Immunohistochemistry-Paraffin: alpha COP I Antibody (3W6Y6) [NBP3-15839] - Immunohistochemistry of paraffin-embedded mouse kidney using alpha COP I Rabbit mAb (NBP3-15839) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839]

Western Blot: alpha COP I Antibody (3W6Y6) [NBP3-15839] - Western blot analysis of extracts of Rat brain, using alpha COP I Rabbit mAb (NBP3-15839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for alpha COP I Antibody (3W6Y6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha COP I

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Alternate Names

alpha coat protein, Alpha-coat protein, Alpha-COP, coatomer protein complex, subunit alpha, coatomer subunit alpha, FLJ26320, HEPCOP, HEP-COPalpha-COP, xenin

Gene Symbol

COPA

Additional alpha COP I Products

Product Documents for alpha COP I Antibody (3W6Y6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha COP I Antibody (3W6Y6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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