alpha Endosulfine Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00273

Novus Biologicals, part of Bio-Techne
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NBP3-00273-100ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha Endosulfine Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human alpha Endosulfine (Accession#: O43768-1; Met1-Glu121).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for alpha Endosulfine Antibody

Western Blot: alpha Endosulfine Antibody [NBP3-00273]

Western Blot: alpha Endosulfine Antibody [NBP3-00273]

Western Blot: alpha Endosulfine Antibody [NBP3-00273] - Anti-alpha Endosulfine rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Whole Cell Lysate. Lane B: Rat heart tissue lysate Whole Cell Lysate. Lane C: Mouse brain tissue lysate Whole Cell Lysate. Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 26 kDa
Immunoprecipitation: alpha Endosulfine Antibody [NBP3-00273]

Immunoprecipitation: alpha Endosulfine Antibody [NBP3-00273]

Immunoprecipitation: alpha Endosulfine Antibody [NBP3-00273] - alpha Endosulfine was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-alpha Endosulfine rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-alpha Endosulfine rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 13 kDa. Observed band size: 15 kDa

Applications for alpha Endosulfine Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Endosulfine

alpha Endosulfine is encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. alpha Endosulfine is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed.

Alternate Names

alpha-endosulfine, ARPP-19eMGC4319, endosulfine alpha, MGC78563, MGC8394

Gene Symbol

ENSA

Additional alpha Endosulfine Products

Product Documents for alpha Endosulfine Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha Endosulfine Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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