Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C2 + C3 + MBS-12

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

Recombinant full-length human alpha-Fetoprotein/AFP protein (Uniprot: P02771)

Localization

Cytoplasmic

Specificity

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Marker

Hepatocellular/Germ Cell Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45011).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013]

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013]

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013] - Immunofluorescence Analysis of PFA-fixed HepG2 cells labeling AFP using alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C2 + C3 + MBS-12) - Azide and BSA Free [NBP2-48013] - Human Fetal Liver stained with AFP Monoclonal Antibody (C2 + C3 + MBS-12).

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Alternate Names

AFP, DSCAM2

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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