Alpha Fodrin Antibody (3D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92689

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D7

Concentration

1 mg/ml

View all Alpha Fodrin Antibodies »

Product Specifications

Immunogen

Recombinant construct (expressed in and purified from E. coli) containing the 19th and 20th spectrin repeats and C-terminal EF hand domains of human alpha Fodrin (amino acids 2086-2447) [UniProt# Q13813].

Localization

Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Note: Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alpha Fodrin Antibody (3D7)

Western Blot: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Western Blot: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Western Blot: Alpha Fodrin Antibody (3D7) [NBP1-92689] - Analysis of neural tissue and cell lysates using mouse mAb to alpha-II spectrin (NBP1-92689, green). [1] protein standard, [2] rat whole brain, [3] rat spinal cord, [4] mouse whole brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, [10] C6 cells. A prominent band at about 250-260 kDa represents the intact alpha-II spectrin heavy chain in neural tissues or cell lines, but not in HeLa.
Immunocytochemistry/ Immunofluorescence: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Immunocytochemistry/ Immunofluorescence: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Immunocytochemistry/Immunofluorescence: Alpha Fodrin Antibody (3D7) [NBP1-92689] - Confocal image of mixed neuron-glial cultures stained with NBP1-92689 antibody to Alpha Fodrin (green) and counterstained for DNA (blue). The Alpha Fodrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. NBP1-92689 clearly reveals the submembraneous cytoskeleton. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. This antibody also reveals the submembranous cytoskeleton of the axon.
Immunohistochemistry: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Immunohistochemistry: Alpha Fodrin Antibody (3D7) [NBP1-92689]

Immunohistochemistry: Alpha Fodrin Antibody (3D7) [NBP1-92689] - Immunofluorescent image of rat cerebellum stained with NBP1-92689 antibody to alpha-II spectrin, dilution 1:2,000, in green, chicken polyclonal antibody to GFAP, dilution 1:5,000, in red. The spectrin antibody stains the submembraneous cytoskeleton on neurons and strongly reveals the cell bodies and dendrites of Purkinje cells, while the GFAP antibody stains the processes of Bergmann glia and astrocytes.

Applications for Alpha Fodrin Antibody (3D7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500

Western Blot

1:3000
Application Notes
This Alpha Fodrin (3D7) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 240 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alpha Fodrin

Alpha Fodrin (SPTAN1; also called non-erythroid alpha spectrin) belongs to spectrin family of proteins which are widely distributed filamentous proteins implicated in generating a supporting/organizing scaffold for cell membranes, and SPTAN1 protein is involved in processes such as secretion, interactions with calmodulin in Ca2+ dependent manner and Ca2+ dependent cytoskeletal movements at the membranes. SPTAN1 interacts with several proteins including ACP1, CALM, EMD, TRPC4 etc. and forms a component of a complex that contains several other proteins such as ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. SPTAN1 cleavage accompanies apoptosis, induced by activation via CD3/TCR complex, Fas/CD95 ligation and other Fas-expressing cells or through cellular exposure to staurosporine, dexamethasone, synthetic ceramide etc. Major products of SPTAN1 cleavage are N-terminal/C-terminal fragments, and phosphorylation of Tyr-1176 has been shown to decrease its sensitivity to cleavage by calpain in vitro. Defects in SPTAN1 have been linked to EIEE5 (epileptic encephalopathy early infantile type 5).

Alternate Names

Alpha-II spectrin, EIEE5, FLJ17738, FLJ44613, Fodrin alpha chain, NEAS, spectrin alpha chain, brain, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spectrin, non-erythroid alpha chain, SPTA2

Entrez Gene IDs

6709 (Human)

Gene Symbol

SPTAN1

UniProt

Q13813 (Human)

Additional Alpha Fodrin Products

Product Documents for Alpha Fodrin Antibody (3D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Alpha Fodrin Antibody (3D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.