Alpha Fodrin Antibody (9H5V8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16131

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16131-100ul
NBP3-16131-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H5V8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alpha Fodrin Antibodies »

Product Summary for Alpha Fodrin Antibody (9H5V8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alpha Fodrin (Q13813). MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alpha Fodrin Antibody (9H5V8)

Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131] - Analysis of extracts of various cell lines, using Alpha Fodrin Rabbit mAb (NBP3-16131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Immunohistochemistry-Paraffin: Alpha Fodrin Antibody (9H5V8) [NBP3-16131] - Mouse kidney using Alpha Fodrin Rabbit mAb (NBP3-16131) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131]

Western Blot: Alpha Fodrin Antibody (9H5V8) [NBP3-16131] - Analysis of extracts of HeLa cells, using Alpha Fodrin Rabbit mAb (NBP3-16131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for Alpha Fodrin Antibody (9H5V8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alpha Fodrin

SPTAN1/Alpha II-spectrin is one of two polypeptide chains (alpha and beta) that are part of the fodrin tetramer. Fodrin functions to link actin filaments to the plasma membrane and is a major neuronal cytoskeletal protein. Fodrin has been linked to neurogenerative and autoimmune diseases.

Alternate Names

Alpha-II spectrin, EIEE5, FLJ17738, FLJ44613, Fodrin alpha chain, NEAS, spectrin alpha chain, brain, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spectrin, non-erythroid alpha chain, SPTA2

Gene Symbol

SPTAN1

Additional Alpha Fodrin Products

Product Documents for Alpha Fodrin Antibody (9H5V8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alpha Fodrin Antibody (9H5V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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