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alpha-Galactosidase A/GLA Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90047

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90047-100ul
NBP2-90047-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for alpha-Galactosidase A/GLA Antibody (001)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human alpha-Galactosidase A/GLA (Accession#: NP_000160.1; Met 1-Leu 429).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for alpha-Galactosidase A/GLA Antibody (001)

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody (001) [NBP2-90047]

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody (001) [NBP2-90047]

Immunoprecipitation: alpha-Galactosidase A/GLA Antibody (001) [NBP2-90047] - Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-GLA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GLA rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size: 52 kDa

Applications for alpha-Galactosidase A/GLA Antibody (001)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 ul/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Galactosidase A/GLA

Galactosidase alpha encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Alternate Names

Agalsidase alpha, GALA, Melibiase

Gene Symbol

GLA

Additional alpha-Galactosidase A/GLA Products

Product Documents for alpha-Galactosidase A/GLA Antibody (001)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for alpha-Galactosidase A/GLA Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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