alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28884

Novus Biologicals, part of Bio-Techne
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NBP1-28884-0.025ml
NBP1-28884-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2A10F3

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human alpha-Methylacyl-CoA Racemase/AMACR expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

Specific for AMACR.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3)BSA Free [NBP1-28884]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3)BSA Free [NBP1-28884]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free [NBP1-28884]

Immunocytochemistry/ Immunofluorescence: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free [NBP1-28884]

Immunocytochemistry/Immunofluorescence: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Confocal immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free [NBP1-28884]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free [NBP1-28884]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis of human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.
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Applications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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