alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07592

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # AMACR/4572R

Concentration

0.2 mg/ml

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Summary for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

Immunogen

Recombinant full-length human alpha-Methylacyl-CoA Racemase/AMACR protein (Uniprot: Q9UHK6)

Localization

Cytoplasmic

Marker

Prostate Cancer Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08468)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592] - Western Blot Analysis of human kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Recombinant Rabbit Monoclonal Antibody (alpha-Methylacyl-CoA Racemase/AMACR/4572R).
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with alpha-Methylacyl-CoA Racemase/AMACR Recombinant Rabbit Monoclonal Antibody (alpha-Methylacyl-CoA Racemase/AMACR/4572R).
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R) [NBP3-07592] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with alpha-Methylacyl-CoA Racemase/AMACR Recombinant Rabbit Monoclonal Antibody (alpha-Methylacyl-CoA Racemase/AMACR/4572R).

Applications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

UniProt

Q9UHK6 (Human)

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/4572R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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