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alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20239

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # AMACR/8350R

Concentration

0.2 mg/ml

Product Summary for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

Immunogen

Recombinant full-length human alpha-Methylacyl-CoA Racemase/AMACR protein

Localization

Cytoplasm (granular)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human prostate adenocarcinoma or renal cell carcinoma (IHC).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/8350R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using alpha-Methylacyl-CoA Racemase/AMACR antibody (AMACR/8350R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for alpha-Methylacyl-CoA Racemase/AMACR Antibody (AMACR/8350R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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