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alpha-N-acetylgalactosaminidase/NAGA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84318

Novus Biologicals, part of Bio-Techne
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NBP1-84318
NBP1-84318-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFST

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-N-acetylgalactosaminidase/NAGA Antibody

Western Blot: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Western Blot: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Western Blot: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318] - Analysis in human cell line TD47D.
Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318]

Immunohistochemistry-Paraffin: alpha-N-acetylgalactosaminidase/NAGA Antibody [NBP1-84318] - Analysis in human placenta and skeletal muscle tissues. Corresponding alpha-N-acetylgalactosaminidase/NAGA RNA-seq data are presented for the same tissues.

Applications for alpha-N-acetylgalactosaminidase/NAGA Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-N-acetylgalactosaminidase/NAGA

NAGA, also known as alpha-N-acetylgalactosaminidase and alpha-galactosidase B, is a member of the glycosyl hydrolase 27 family. NAGA plays an important role in the breakdown of glycolipids by removing the terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Additionally, NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase. Defects in the NAGA gene have been identified as the cause of Schindler type I and type II disease.

Alternate Names

alphaNacetylgalactosaminidase, NAGA

Gene Symbol

NAGA

Additional alpha-N-acetylgalactosaminidase/NAGA Products

Product Documents for alpha-N-acetylgalactosaminidase/NAGA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for alpha-N-acetylgalactosaminidase/NAGA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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