alpha-Sarcoglycan Antibody (3C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006442-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha-Sarcoglycan Antibodies »

Product Specifications

Immunogen

SGCA (NP_000014.1, 26 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TTLHPLVGRVFVHTLDHETFLSLPEHVAVPPAVHITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLP

Specificity

SGCA - sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (3C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alpha-Sarcoglycan Antibody (3C4)

Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01]

Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01]

Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Analysis of SGCA expression in transfected 293T cell line by SGCA monoclonal antibody (M01), clone 3C4.Lane 1: SGCA transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Detection limit for recombinant GST tagged SGCA is 1 ng/ml as a capture antibody.

Applications for alpha-Sarcoglycan Antibody (3C4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Alternate Names

50-DAG, Adhalin, ADL, alphaSarcoglycan, DAG2, DMDA2, Dystroglycan-2, LGMD2D, SCARMD1, SGCA

Entrez Gene IDs

6442 (Human)

Gene Symbol

SGCA

UniProt

NP_000014

Additional alpha-Sarcoglycan Products

Product Documents for alpha-Sarcoglycan Antibody (3C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Sarcoglycan Antibody (3C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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