alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34522
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Monkey, Rabbit, Sheep
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1A4/asm-1
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Alpha-Smooth Muscle Actin Antibody (1A4/asm-1) is made to the N-Terminal decapeptide of Alpha smooth muscle isoform of actin and conjugated to KLH.
Reactivity Notes
Feline reactivity reported from a verified customer review.
Localization
Cytoplasmic
Specificity
Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Marker
Leiomyosarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free
Western Blot Detection of alpha-Smooth Muscle Actin in Human Heart Tissue and iBJ6 hiPSCs
Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 ug/mL of Mouse Anti-alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog # NBP2-33006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). The observed molecular weight was detected for alpha Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions. Image using the standard format of this product.
Immunohistological Staining of alpha-Smooth Muscle Actin in Paraffin Embedded Cancer Tissues
M2 macrophage-related protein expression and the co-location of M2-macrophages and GC-MSCs within tumor tissues. Representative immunofluorescence images of the co-location of GC-MSCs (alpha-Smooth Muscle Actin; green) and M2-macrophages (CD204; red) in gastric cancer tissues detected by confocal microscopy. scale bar, 100 um. **P < 0.01 vs. gastric cancer tissue. T: tumor; N: non-cancerous tissues. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-019-2131-y), licensed under a CC-BY license. Image using the standard format of this product.
Flow Cytometry Analysis of alpha-Smooth Muscle Actin in Colorectal Tumor Cells using Allophycocyanin Conjugated Antibody
alpha-Smooth Muscle Actin expression in colorectal tumor cells. Using Allophycocyanin conjugated version of antibody (NBP2-34522APC). Image from verified customer review.
Applications for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5ug/ml for coating
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Frozen
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Immunoprecipitation
0.5-1ug/500ug protein lysate
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34276989).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha-Smooth Muscle Actin
ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).
References
1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449
2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004
3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Additional alpha-Smooth Muscle Actin Products
Product Documents for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free
Product Specific Notices for alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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