alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37641

Novus Biologicals, part of Bio-Techne
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NBP2-37641-0.025ml
NBP2-37641
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all alpha-Smooth Muscle Actin Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human alpha-Smooth Muscle Actin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8)BSA Free [NBP2-37641]

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8)BSA Free [NBP2-37641]

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis using alpha-Smooth Muscle Actin Antibody (1H8) against Hela (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate. Bands indicate an observed molecular weight of ~42 kDa.
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (1H8) (green) and negative control (purple).
View 1 more images

Applications for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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