alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37641
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1H8
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human alpha-Smooth Muscle Actin expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free
Western Blot: alpha-Smooth Muscle Actin Antibody (1H8)BSA Free [NBP2-37641]
Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis using alpha-Smooth Muscle Actin Antibody (1H8) against Hela (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate. Bands indicate an observed molecular weight of ~42 kDa.Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]
Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (1H8) (green) and negative control (purple).Applications for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha-Smooth Muscle Actin
ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).
References
1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449
2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004
3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Additional alpha-Smooth Muscle Actin Products
Product Documents for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free
Product Specific Notices for alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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