alpha-Smooth Muscle Actin Antibody (BLR082G)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78836

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-78836
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR082G

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all alpha-Smooth Muscle Actin Antibodies »

Product Specifications

Immunogen

Alpha-Smooth Muscle Actin Antibody (BLR082G) is a recombinant monoclonal made to a peptide representing a region between residue between 1 and 50 of human aortic smooth muscle actin using the numbering given in entry NP_001135417.1 (Gene ID 59).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (BLR082G)

Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE IMR-90 cells by ICC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE breast by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of mouse alpha-Smooth Muscle Actin in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for alpha-Smooth Muscle Actin Antibody (BLR082G)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Borate Buffered Saline (BBS) pH 8.2

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA, 1a4 sma, 1a4 smooth muscle actin, alpha sma 1a4, anti-alpha SMA, sma 1a4

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (BLR082G)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody (BLR082G)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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