alpha-Smooth Muscle Actin Antibody (SPM332) [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34760G
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Monkey, Rabbit, Sheep
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM332
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH. (Uniprot: P62736)
Localization
Cytoplasmic
Specificity
Actin is a major component of the cytoskeleton and is present in most cell types. This monoclonal antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This monoclonal antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Marker
Leiomyosarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Applications for alpha-Smooth Muscle Actin Antibody (SPM332) [DyLight 488]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: alpha-Smooth Muscle Actin
ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).
References
1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449
2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004
3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Additional alpha-Smooth Muscle Actin Products
Product Documents for alpha-Smooth Muscle Actin Antibody (SPM332) [DyLight 488]
Product Specific Notices for alpha-Smooth Muscle Actin Antibody (SPM332) [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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