alpha Tubulin 4a Antibody (2E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00007277-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha Tubulin 4a Antibodies »

Product Specifications

Immunogen

TUBA1 (AAH09238, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

Specificity

TUBA1 - tubulin, alpha 1 (testis specific)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alpha Tubulin 4a Antibody (2E11)

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - TUBA1 monoclonal antibody (M01), clone 2E11. Analysis of TUBA1 expression in different Cell lines and Human Tissue.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (2E11) [H00007277-M01]

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
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Applications for alpha Tubulin 4a Antibody (2E11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Tubulin 4a

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.

Long Name

Tubulin alpha-4A chain

Alternate Names

Alpha-tubulin 1, TUBA1, TUBA4A, Tubulin H2-alpha

Entrez Gene IDs

7277 (Human)

Gene Symbol

TUBA4A

OMIM

191110 (Human)

UniProt

AAH09238 (Human)

Additional alpha Tubulin 4a Products

Product Documents for alpha Tubulin 4a Antibody (2E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha Tubulin 4a Antibody (2E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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