Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chicken, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B-5-1-2

Format

BSA Free

Concentration

2.0 mg/ml

View all alpha Tubulin 4a Antibodies »

Product Specifications

Immunogen

Sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin).

Reactivity Notes

Cross-reacts with African Green Monkey, Chlamydomonas reinhardtii, kangaroo rat and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Recognizes an epitope located at the C-terminal end of the alpha-tubulin isoform in a variety of organisms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

~50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

Western Blot: alpha Tubulin 4a Antibody (B-5-1-2)BSA Free [NB120-11304]

Western Blot: alpha Tubulin 4a Antibody (B-5-1-2)BSA Free [NB120-11304]

Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) HeLa, 2.) JURKAT, 3.) COS7, 4.) NIH-3T3, 5.) PC-12, 6.) RAT2, 7.) CHO, 8.) MDBK, 9.) MDCK
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304]

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and Anti-Mouse Alexa543 secondary antibody. Microtubules detected with Anti-alpha-tubulin Mouse primary antibody and Anti-Mouse Alexa488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304]

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-alpha-Tubulin antibody produced in mouse clone No. B-5-1-2. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.
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Applications for alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

0.25-0.5 ug/ml
Application Notes
ICC: Concentration is recommended using cultured chicken fibroblasts (CFB). WB: Concentration is recommended using a total cell extract of the human foreskin fibroblast cell line (FS11). Predicted molecular weight: 50 kDa.
See Simple Western Antibody Database for Simple Western validation: tested in Caco2 cell lysates; antibody dilution of 1:50; separated by size

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Tubulin 4a

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody.

Long Name

Tubulin alpha-4A chain

Alternate Names

Alpha-tubulin 1, TUBA1, TUBA4A, Tubulin H2-alpha

Entrez Gene IDs

7277 (Human); 22143 (Mouse); 500929 (Rat)

Gene Symbol

TUBA4A

Additional alpha Tubulin 4a Products

Product Documents for alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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