alpha Tubulin Antibody (RM113) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18536
Recombinant Monoclonal
Key Product Details
Species Reactivity
All Species
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM113
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This alpha Tubulin Antibody (RM113) was developed against a peptide corresponding to the C-terminus of alpha Tubulin
Specificity
This alpha Tubulin Antibody (RM113) reacts to alpha Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for alpha Tubulin Antibody (RM113) [Biotin]
Western Blot: alpha Tubulin Antibody (RM113) [Biotin] [NBP3-18536] - Western blot of A431 cells, using NBP3-18536 at 1/1000 dilution, showed a band of alpha Tubulin at the predicted MW (~50 kDa).
Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (RM113) [Biotin] [NBP3-18536] - Immunocytochemical staining of HeLa cells, using NBP3-18536 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
Applications for alpha Tubulin Antibody (RM113) [Biotin]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:200
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:200
Immunoprecipitation
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol, PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (RM113) [Biotin]
Product Specific Notices for alpha Tubulin Antibody (RM113) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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