alpha Tubulin Antibody (RM113)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77401

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-77401
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Conjugate
Catalog #
Biotin
NBP3-18536
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Key Product Details

Species Reactivity

Validated:

All Species

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM113

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha Tubulin Antibodies »

Product Summary for alpha Tubulin Antibody (RM113)

Immunogen

This alpha Tubulin Antibody (RM113) was developed against a peptide corresponding to the C-terminus of alpha Tubulin

Specificity

This alpha Tubulin Antibody (RM113) reacts to alpha Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin Antibody (RM113)

Western Blot: alpha Tubulin Antibody (RM113) [NBP2-77401]

Western Blot: alpha Tubulin Antibody (RM113) [NBP2-77401]

Western Blot: alpha Tubulin Antibody (RM113) [NBP2-77401] - A431 cells, using NBP2-77401, showed a band of alpha-Tubulin at the predicted molecular weight of ~50 kDa.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (RM113) [NBP2-77401]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (RM113) [NBP2-77401]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (RM113) [NBP2-77401] - Staining of HeLa cells, using NBP2-77401 (red). Nuclei have been labeled with DAPI (blue).

Applications for alpha Tubulin Antibody (RM113)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:200

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Gene Symbol

TUBA1A

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (RM113)

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Certificate of Analysis

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Product Specific Notices for alpha Tubulin Antibody (RM113)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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