alpha Tubulin Antibody (TU-01) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-333

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Mammal, Plant, Turkey, Yeast

Cited:

Plant

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-01

Format

BSA Free

Concentration

1.0 mg/ml

View all alpha Tubulin Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (TU-01) was developed against a fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26594217).

Localization

Major constituent of microtubules.

Specificity

This alpha Tubulin Antibody (TU-01) recognizes the defined epitope (aa 65-97) on N-terminal structureal domain of alpha tubulin.

Marker

Microtubule Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin Antibody (TU-01) - BSA Free

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).
Immunoprecipitation: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunoprecipitation: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunoprecipitation: alpha Tubulin Antibody (TU-01) [NB500-333] - alpha Tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Molecular weight of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)
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Applications for alpha Tubulin Antibody (TU-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
Western blotting: Recommended dilution in reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB500-333 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Entrez Gene IDs

20766 (Mouse)

Gene Symbol

TUBA1A

UniProt

P02550 (Porcine)

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (TU-01) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha Tubulin Antibody (TU-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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