AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20407
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CRYAB/4663
Concentration
0.2 mg/ml
Product Summary for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Immunogen
Recombinant human full-length AlphaB Crystallin/CRYAB protein
Reactivity Notes
Others-not tested
Localization
Cytoplasm, translocates to nucleus during heat shock and resides in nuclear splicing speckles.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: 293T whole cell lysates; heart and brain.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CRYAB/4663).
AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Formalin-fixed, paraffin-embedded human heart stained with AlphaB Crystallin/CRYAB antibody (CRYAB/4663). Inset: PBS instead of primary antibody; secondary only negative control.
AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Formalin-fixed, paraffin-embedded human prostate cancer stained with AlphaB Crystallin/CRYAB antibody (CRYAB/4663). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AlphaB Crystallin/CRYAB
Alternate Names
CRYA2, CRYAB, CTPP2, HSPB5
Gene Symbol
CRYAB
Additional AlphaB Crystallin/CRYAB Products
Product Documents for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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