Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunomicroscopy, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all AMBRA1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]

Localization

Cytoplasmic vesicle, autophagosome.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMBRA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - Double labeling for Ambra1 (green) and PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 um). The higher magnification image (scale bar, 5 um) from the stratum pyramidale shows a PV neuron (right; merge in orange) and an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity and neuron borders are indicated by a dash line (n=3 mice; three sections per animal). Image collected and cropped by CiteAb from the following publication (link.springer.com/article/10.1007/s12035-018-0911-5), licensed under a CC-BY license.
Western Blot: AMBRA1 AntibodyBSA Free [NBP1-07124]

Western Blot: AMBRA1 AntibodyBSA Free [NBP1-07124]

Western Blot: AMBRA1 Antibody [NBP1-07124] - WB analysis of AMBRA1 in mouse liver cell lysate.
Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.
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Applications for AMBRA1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Western Blot

1:1000
Application Notes
This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMBRA1

AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.

Alternate Names

activating molecule in BECN1-regulated autophagy protein 1, autophagy/beclin-1 regulator 1, DCAF3, DDB1 and CUL4 associated factor 3, FLJ20294, KIAA1736activating molecule in beclin-1-regulated autophagy, MGC33725, WD repeat domain 94, WDR94

Entrez Gene IDs

55626 (Human); 228361 (Mouse); 59319 (Rat)

Gene Symbol

AMBRA1

UniProt

A2AH22 (Mouse)

Additional AMBRA1 Products

Product Documents for AMBRA1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMBRA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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