I would like to use the antibody (Cat.No. NBP1-07124) with chicken samples. Could you align the immunogen of this antibody to check, if any or all of the predicted chicken isoforms show homology with the Immunizing peptide?

Name: AMBRA1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-07124

We ran alignment of chicken's AMBRA1 sequence with the immunogen of NBP1-07124, and found that this antibody will not be able to detect this target in chicken samples.

AMBRA1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-07124

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Citations (6)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP1-07124AF350

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Alexa Fluor 405

NBP1-07124AF405

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Alexa Fluor 488

NBP1-07124AF488

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Alexa Fluor 532

NBP1-07124AF532

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Alexa Fluor 594

NBP1-07124AF594

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Alexa Fluor 647

NBP1-07124AF647

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Alexa Fluor 700

NBP1-07124AF700

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Alexa Fluor 750

NBP1-07124AF750

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Allophycocyanin

NBP1-07124APC

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Biotin

NBP1-07124B

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DyLight 350

NBP1-07124UV

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DyLight 405

NBP1-07124V

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DyLight 488

NBP1-07124G

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DyLight 550

NBP1-07124R

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DyLight 594

NBP1-07124DL594

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DyLight 650

NBP1-07124C

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DyLight 680

NBP1-07124FR

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DyLight 755

NBP1-07124IR

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FITC

NBP1-07124F

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HRP

NBP1-07124H

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Janelia Fluor 525

NBP1-07124JF525

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Janelia Fluor 549

NBP1-07124JF549

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Janelia Fluor 585

NBP1-07124JF585

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Janelia Fluor 635

NBP1-07124JF635

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Janelia Fluor 646

NBP1-07124JF646

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Janelia Fluor 669

NBP1-07124JF669

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NBP1-07124PE

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PerCP

NBP1-07124PCP

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunomicroscopy, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all AMBRA1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]

Localization

Cytoplasmic vesicle, autophagosome.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMBRA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - Double labeling for Ambra1 (green) and PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 um). The higher magnification image (scale bar, 5 um) from the stratum pyramidale shows a PV neuron (right; merge in orange) and an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity and neuron borders are indicated by a dash line (n=3 mice; three sections per animal). Image collected and cropped by CiteAb from the following publication (link.springer.com/article/10.1007/s12035-018-0911-5), licensed under a CC-BY license.

Western Blot: AMBRA1 AntibodyBSA Free [NBP1-07124]

Western Blot: AMBRA1 Antibody [NBP1-07124] - WB analysis of AMBRA1 in mouse liver cell lysate.

Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - AMBRA1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse epidermis using DAB with hematoxylin counterstain.

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] -

The protein levels of AMBRA1, LC3 and SQSTM1 were analyzed by western blot from fresh tissue samples. Tubulin was also detected as the control of sample loading. Representative western blots were shown. N normal tissue, T PCa tissue.

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] -

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] - a Immunohistochemical results showing the prevalence of AMBRA1 (left) & SQSTM1 (right) based on a semi-quantitative total score. Frequency distribution of the protein is reported according to the Gleason grade classification (GL6–GL8). A trend of AMBRA1 high score values correlation with the higher grade of Gleason score is visible. As opposite the levels of SQSTM1 positivity appeared higher in tumors with a lower Gleason score (Gleason 6). b The protein levels of AMBRA1, LC3 & SQSTM1 were analyzed by western blot from fresh tissue samples. Tubulin was also detected as the control of sample loading. Representative western blots were shown. N normal tissue, T PCa tissue. c mRNA expression of AMBRA1 & SQSTM1 clearly shows that a significant upregulation of these genes occurs in PCa in comparison with BPH. * P < 0.01; ° P < 0.05 Image collected & cropped by CiteAb from the following publication (http://link.springer.com/10.1007/s10495-015-1176-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for AMBRA1 Antibody - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Western Blot

1:1000

Application Notes

This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMBRA1

AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.

Alternate Names

activating molecule in BECN1-regulated autophagy protein 1, autophagy/beclin-1 regulator 1, DCAF3, DDB1 and CUL4 associated factor 3, FLJ20294, KIAA1736activating molecule in beclin-1-regulated autophagy, MGC33725, WD repeat domain 94, WDR94

Entrez Gene IDs

55626 (Human); 228361 (Mouse); 59319 (Rat)

Gene Symbol

AMBRA1

UniProt

A2AH22 (Mouse)

Additional AMBRA1 Products

Product Documents for AMBRA1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Protocol

COA

SDS

Download SDS

Product Specific Notices for AMBRA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

AMBRA1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-07124

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all AMBRA1 Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Scientific Data Images for AMBRA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Western Blot: AMBRA1 AntibodyBSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody - BSA Free [NBP1-07124]

Immunohistochemistry: AMBRA1 Antibody - BSA Free [NBP1-07124]

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] -

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] -

Applications for AMBRA1 Antibody - BSA Free

ELISA

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: AMBRA1

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional AMBRA1 Products

Product Documents for AMBRA1 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for AMBRA1 Antibody - BSA Free