Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all AMFR/gp78 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 500 and 550 of human Autocrine Motility Factor Receptor using the numbering given in entry NP_001135.3 (GeneID 267).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMFR/gp78 Antibody

Western Blot: AMFR/gp78 Antibody [NBP1-49986]

Western Blot: AMFR/gp78 Antibody [NBP1-49986]

Western Blot: AMFR/gp78 Antibody [NBP1-49986] - Detection of Human gp78 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-gp78 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. gp78 was also immunoprecipitated by rabbit anti-gp78 antibody BL10542, which recognizes an upstream epitope. For blotting immunoprecipitated gp78 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for AMFR/gp78 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AMFR/gp78

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.

Long Name

Autocrine Motility Factor Receptor

Alternate Names

gp78, RNF45

Gene Symbol

AMFR

Additional AMFR/gp78 Products

Product Documents for AMFR/gp78 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMFR/gp78 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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