Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-BMDM1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Aminopeptidase N/CD13 Antibodies »

Product Summary for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free

Immunogen

Aminopeptidase N/CD13 Antibody (ER-BMDM1) made from Balb/c bone marrow derived macrophages.

Localization

Cell membrane; single-pass type II membrane protein

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574] - Staining of a mouse tail cryosection with anti-mouse CD13 antibody. Image from the standard format of this antibody.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574] - Staining of mouse peripheral blood granulocytes with rat anti-mouse CD13. Image from the standard format of this antibody.
Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574]

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free [NBP2-80574] - Mouse lymph node cryosection anti-CD13 antibody. Image from the standard format of this antibody.
View 1 more images

Applications for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:50

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500
Application Notes
For Flow Cytometry: Use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase N/CD13

Human CD13 is expressed on the majority of peripheral blood monocytes and granulocytes. It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses (HCV).

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aminopeptidase N/CD13 Antibody (ER-BMDM1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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