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Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55358

Clone WM15 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB110-55358
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # WM15

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Immunogen

Human AML cells (NM_001150.2).

Specificity

The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.

Applications for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Application
Recommended Usage

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Aminopeptidase N/CD13

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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