Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16626

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16626-100ul
NBP3-16626-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6V8C0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Aminopeptidase PILS/ARTS1 Antibodies »

Product Summary for Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 842-941 of human Aminopeptidase PILS/ARTS1 (Q9NZ08). NPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQTIETIEENIGWMDKNFDKIRVWLQSEKLERM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded human placenta using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626]

Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of Rat liver, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminopeptidase PILS/ARTS1

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon-gamma. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Gene Symbol

ERAP1

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aminopeptidase PILS/ARTS1 Antibody (6V8C0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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