Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Aminopeptidase PILS/ARTS1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP. The immunogen is located within amino acids 730 - 780 of ERAP1 . Amino Acid Squence: VQRAEGYFRKWKESNGNL

Specificity

At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase PILS/ARTS1 Antibody - BSA Free

Western Blot: Aminopeptidase PILS/ARTS1 AntibodyBSA Free [NBP2-41232]

Western Blot: Aminopeptidase PILS/ARTS1 AntibodyBSA Free [NBP2-41232]

Western Blot: Aminopeptidase PILS/ARTS1 Antibody [NBP2-41232] - Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 ug/ml.
Aminopeptidase PILS/ARTS1 Antibody - BSA Free

Immunohistochemistry: Aminopeptidase PILS/ARTS1 Antibody - BSA Free [NBP2-41232] -

Immunohistochemistry: Aminopeptidase PILS/ARTS1 Antibody - BSA Free [NBP2-41232] - Immunohistochemistry of Aminopeptidase PILS/ARTS1 in mouse brain tissue with Aminopeptidase PILS/ARTS1 antibody at 5 u/mL.
Aminopeptidase PILS/ARTS1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Aminopeptidase PILS/ARTS1 Antibody - BSA Free [NBP2-41232] -

Immunocytochemistry/ Immunofluorescence: Aminopeptidase PILS/ARTS1 Antibody - BSA Free [NBP2-41232] - Immunofluorescence of Aminopeptidase PILS/ARTS1 in mouse brain tissue with Aminopeptidase PILS/ARTS1 antibody at 20 ug/mL.

Applications for Aminopeptidase PILS/ARTS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase PILS/ARTS1

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon-gamma. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Gene Symbol

ERAP1

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Aminopeptidase PILS/ARTS1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aminopeptidase PILS/ARTS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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