Amphiregulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15379

Novus Biologicals, part of Bio-Techne
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NBP2-15379
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Amphiregulin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Amphiregulin. The exact sequence is proprietary.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Amphiregulin Antibody

Western Blot: Amphiregulin Antibody [NBP2-15379]

Western Blot: Amphiregulin Antibody [NBP2-15379]

Western Blot: Amphiregulin Antibody [NBP2-15379] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Amphiregulin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP2-15379]

Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP2-15379]

Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP2-15379] - Paraffin-embedded human colon cancer. Amphiregulin antibody diluted at 1:500.

Applications for Amphiregulin Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Amphiregulin

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq]

Alternate Names

AREG

Gene Symbol

AREG

UniProt

P15514

Additional Amphiregulin Products

Product Documents for Amphiregulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Amphiregulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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