AMPK alpha 1 Antibody (2B7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80575
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80575-img0005.jpg "Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]")
![Western Blot: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Western-Blot-NBP2-80575-img0001.jpg "Western Blot: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2B7
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free
Western Blot: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]
Western Blot: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80575-img0002.jpg "Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]")
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]
Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.![Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80575-img0003.jpg "Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]")
Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]
Immunohistochemistry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining. Image from the standard format of this antibody.![Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80575-img0004.jpg "Flow Cytometry: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]")
![ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-ELISA-NBP2-80575-img0006.jpg "ELISA: AMPK alpha 1 Antibody (2B7) - Azide and BSA Free [NBP2-80575]")
![Simple Western: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]](https://resources.bio-techne.com/images/products/AMPK-alpha-1-Antibody-2B7-Azide-and-BSA-Free-Simple-Western-NBP2-80575-img0007.jpg "Simple Western: AMPK alpha 1 Antibody (2B7)Azide and BSA Free [NBP2-80575]")
Applications for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Simple Western
1:50
Western Blot
1:500
Application Notes
This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AMPK alpha 1
Long Name
AMP-activated Protein Kinase alpha 1
Alternate Names
PRKAA1
Gene Symbol
PRKAA1
Additional AMPK alpha 1 Products
Product Documents for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free
Product Specific Notices for AMPK alpha 1 Antibody (2B7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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