Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation

Label

Agarose

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22767231).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for AMPK alpha 1 Antibody [Agarose]

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
Immunoprecipitation - 15-25 ul gel slurry per 0.1-1mg protein lysate or extract. Antibody was affinity purified using an epitope specific to AMPK alpha 1 immobilized on solid support.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AMPK alpha 1

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Entrez Gene IDs

5562 (Human); 105787 (Mouse); 65248 (Rat)

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody [Agarose]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMPK alpha 1 Antibody [Agarose]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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