AMPK beta 2/PRKAB2 Antibody (2G9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005565-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2G9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AMPK beta 2 Antibodies »

Product Specifications

Immunogen

PRKAB2 (AAH53610, 1 a.a. ~ 272 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI

Specificity

PRKAB2 - protein kinase, AMP-activated, beta 2 non-catalytic subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AMPK beta 2/PRKAB2 Antibody (2G9)

Western Blot: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01]

Western Blot: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01]

Western Blot: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01] - PRKAB2 monoclonal antibody (M01), clone 2G9 Analysis of PRKAB2 expression in Hela.
Sandwich ELISA: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01] - Detection limit for recombinant GST tagged PRKAB2 is approximately 10ng/ml as a capture antibody.

Applications for AMPK beta 2/PRKAB2 Antibody (2G9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AMPK beta 2

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Long Name

AMP-activated Protein Kinase beta 2

Alternate Names

PRKAB2

Entrez Gene IDs

5565 (Human)

Gene Symbol

PRKAB2

OMIM

602741 (Human)

UniProt

AAH53610 (Human)

Additional AMPK beta 2 Products

Product Documents for AMPK beta 2/PRKAB2 Antibody (2G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMPK beta 2/PRKAB2 Antibody (2G9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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