Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AMPK beta 2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKAB2 (NP_005390) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK beta 2/PRKAB2 Antibody (OTI4H4) - Azide and BSA Free

Western Blot: AMPK beta 2/PRKAB2 Antibody (OTI4H4)Azide and BSA Free [NBP2-71226]

Western Blot: AMPK beta 2/PRKAB2 Antibody (OTI4H4)Azide and BSA Free [NBP2-71226]

Western Blot: AMPK beta 2/PRKAB2 Antibody (OTI4H4) - Azide and BSA Free [NBP2-71226] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAB2.

Applications for AMPK beta 2/PRKAB2 Antibody (OTI4H4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK beta 2

PRKAB2 is encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. [provided by RefSeq]

Long Name

AMP-activated Protein Kinase beta 2

Alternate Names

PRKAB2

Gene Symbol

PRKAB2

Additional AMPK beta 2 Products

Product Documents for AMPK beta 2/PRKAB2 Antibody (OTI4H4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMPK beta 2/PRKAB2 Antibody (OTI4H4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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