Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31099

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Canine, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 156C135.2

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Androgen R/NR3C4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.

Reactivity Notes

Predicted cross-reactivity with Chimpanzee, Canine, Mouse, Porcine and Rat.

Modification

p Ser210, p Ser213

Specificity

In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images

Western Blot: Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free [NBP2-31099] - LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h. (See Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003 Fig. 2)

Applications

Application
Recommended Usage

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

1:20-1:1000. Use reported in scientific literature (Lin et al (2007))

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Androgen R/NR3C4

The androgen receptor (AR) is an approx. 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790 (Lin et al. 2003). The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

Additional Androgen R/NR3C4 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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