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Angiogenin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41185

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG. The immunogen is located within amino acids 70 - 120 of ANG. Amino Acid Squence: NKNGNPHRENLRISKS

Specificity

Two alternatively spliced transcript variants have been observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiogenin Antibody - BSA Free

Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]

Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]

Western Blot: Angiogenin Antibody [NBP2-41185] - Angiogenin and EPCs modulation after human stroke and rehabilitation. (A) Infarct tissue and contralateral brain homogenates (less than or equal 4 days) were analyzed by western blot for angiogenin (n = 3). The densitometry results (arbitrary units) were corrected by the actin load and bar graph showing the angiogenin fold-change of the ipsilateral vs. contralateral signal. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full) licensed under a CC-BY license.
Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]

Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]

Western Blot: Angiogenin Antibody [NBP2-41185] - Analysis of ANG in rat liver tissue lysate with Ang antibody at (A) 1 and (B) 2 ug/mL.
Angiogenin Antibody - BSA Free

Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] -

Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] - Angiogenin & EPCs modulation after mouse cerebral ischemia & rehabilitation. (A) Infarct & contralateral mouse brain cortical homogenates were analyzed by western blot to quantify angiogenin protein (n = 3). The densitometry results (arbitrary units) were corrected by the actin load & are represented as fold-change of the ipsilateral vs. contralateral signal. (B) Bar graphs representing the angiogenin RNA expression in the ischemic & contralateral cortex in the short-term (n = 6–9) & long-term rehabilitation groups (n = 5), **p < 0.01, *p < 0.05 as indicated by horizontal lines. Non-RHB ipsilateral short-term vs. long-term; &p < 0.05. Pasta Matrix & Treadmill contralateral short-term vs. long-term; †p < 0.05. (C) Bar graphs showing the percentage of the Angiogenin+ area in the pasta matrix long-term rehabilitation group (n = 6) together with images of representative brains with inserts showing co-localization of angiogenin in neurons: scale bar represents 100 μm. *p < 0.05. (D) Graph showing the plasma angiogenin temporal profile of No-RHB, Pasta matrix, & Treadmill groups (n = 3–4/group); *p < 0.05. (E) Box plots representing the cell density of EPCs from the three different RHB groups (n = 3–5 short-term; n = 6–7 long-term) & representative images of the primary cultures; scale bar represents 250 μm. Data are represented as the mean ± SEM or as box plots indicating the median (IQR). IP, ipsilateral; CL, contralateral; ST-R, short-term rehabilitation; LT-R, long-term rehabilitation; No-RHB, No-Rehabilitation; EPC, endothelial progenitor cells. Image collected & cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fneur.2018.00508/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Angiogenin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1 - 2 ug/mL
Application Notes
Use in IHC reported in scientific literature (PMID: 30008694).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiogenin

Angiogenin was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human recombinant angiogenin.

Alternate Names

ANG, RNase 5

Gene Symbol

ANG

Additional Angiogenin Products

Product Documents for Angiogenin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiogenin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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