Angiogenin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41185
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG. The immunogen is located within amino acids 70 - 120 of ANG. Amino Acid Squence: NKNGNPHRENLRISKS
Specificity
Two alternatively spliced transcript variants have been observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Angiogenin Antibody - BSA Free
Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]
Western Blot: Angiogenin Antibody [NBP2-41185] - Angiogenin and EPCs modulation after human stroke and rehabilitation. (A) Infarct tissue and contralateral brain homogenates (less than or equal 4 days) were analyzed by western blot for angiogenin (n = 3). The densitometry results (arbitrary units) were corrected by the actin load and bar graph showing the angiogenin fold-change of the ipsilateral vs. contralateral signal. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00508/full) licensed under a CC-BY license.Western Blot: Angiogenin AntibodyBSA Free [NBP2-41185]
Western Blot: Angiogenin Antibody [NBP2-41185] - Analysis of ANG in rat liver tissue lysate with Ang antibody at (A) 1 and (B) 2 ug/mL.Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] -
Western Blot: Angiogenin Antibody - BSA Free [NBP2-41185] - Angiogenin & EPCs modulation after mouse cerebral ischemia & rehabilitation. (A) Infarct & contralateral mouse brain cortical homogenates were analyzed by western blot to quantify angiogenin protein (n = 3). The densitometry results (arbitrary units) were corrected by the actin load & are represented as fold-change of the ipsilateral vs. contralateral signal. (B) Bar graphs representing the angiogenin RNA expression in the ischemic & contralateral cortex in the short-term (n = 6–9) & long-term rehabilitation groups (n = 5), **p < 0.01, *p < 0.05 as indicated by horizontal lines. Non-RHB ipsilateral short-term vs. long-term; &p < 0.05. Pasta Matrix & Treadmill contralateral short-term vs. long-term; †p < 0.05. (C) Bar graphs showing the percentage of the Angiogenin+ area in the pasta matrix long-term rehabilitation group (n = 6) together with images of representative brains with inserts showing co-localization of angiogenin in neurons: scale bar represents 100 μm. *p < 0.05. (D) Graph showing the plasma angiogenin temporal profile of No-RHB, Pasta matrix, & Treadmill groups (n = 3–4/group); *p < 0.05. (E) Box plots representing the cell density of EPCs from the three different RHB groups (n = 3–5 short-term; n = 6–7 long-term) & representative images of the primary cultures; scale bar represents 250 μm. Data are represented as the mean ± SEM or as box plots indicating the median (IQR). IP, ipsilateral; CL, contralateral; ST-R, short-term rehabilitation; LT-R, long-term rehabilitation; No-RHB, No-Rehabilitation; EPC, endothelial progenitor cells. Image collected & cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fneur.2018.00508/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Angiogenin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Western Blot
1 - 2 ug/mL
Application Notes
Use in IHC reported in scientific literature (PMID: 30008694).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Angiogenin
Alternate Names
ANG, RNase 5
Gene Symbol
ANG
Additional Angiogenin Products
Product Documents for Angiogenin Antibody - BSA Free
Product Specific Notices for Angiogenin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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