Angiopoietin-1 Antibody (SN67-02)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67664

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-67664
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN67-02

Concentration

1 mg/ml

View all Angiopoietin-1 Antibodies »

Product Summary for Angiopoietin-1 Antibody (SN67-02)

Immunogen

Synthetic peptide within Human Angiopoietin-1 aa 249-300 / 498. (SwissProt: Q15389 Human; SwissProt: O35460 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Angiopoietin-1 Antibody (SN67-02)

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664] - Analysis of Angiopoietin 1 on K562 cells lysates using anti-Angiopoietin 1 antibody at 1/1,000 dilution.
Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664] - Western blot analysis of Angiopoietin-1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Applications for Angiopoietin-1 Antibody (SN67-02)

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody (SN67-02)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Angiopoietin-1 Antibody (SN67-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.