Angiopoietin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90169

Novus Biologicals, part of Bio-Techne
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NBP1-90169
NBP1-90169-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Angiopoietin-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SNQRRSPENSGRRYNRIQHGQCAYTFILPEHDGNCRESTTDQYNTNALQRDAPHVEPDFSSQKLQHLEHVME

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Angiopoietin-1 Antibody

Western Blot: Angiopoietin-1 Antibody [NBP1-90169]

Western Blot: Angiopoietin-1 Antibody [NBP1-90169]

Western Blot: Angiopoietin-1 Antibody [NBP1-90169] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody [NBP1-90169]

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody [NBP1-90169]

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody [NBP1-90169] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for Angiopoietin-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Angiopoietin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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