Angiopoietin-2 Antibody (JM71-34)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67154

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM71-34

Concentration

1 mg/ml

View all Angiopoietin-2 Antibodies »

Product Summary for Angiopoietin-2 Antibody (JM71-34)

Immunogen

Synthetic peptide within Human Angiopoietin-2 aa 351-400 / 496. (SwissProt: O15123 Human; SwissProt: O35608 Mouse; SwissProt: O35462 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Angiopoietin-2 Antibody (JM71-34)

Western Blot: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Western Blot: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Western Blot: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Western blot analysis of Angiopoietin-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: human liver tissue lysate Lane 3: human placenta tissue lysate Lane 4: mouse liver tissue lysate
Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154]

Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded human colon cancer tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.
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Applications for Angiopoietin-2 Antibody (JM71-34)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (JM71-34)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Angiopoietin-2 Antibody (JM71-34)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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