Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H7

Concentration

1 mg/ml

View all Angiopoietin-2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 19-496 of human ANGPT2(NP_001138) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-2 Antibody (OTI3H7)

Western Blot: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Western Blot: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Western Blot: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.
Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Immunohistochemistry: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of Human lymphoma tissue
Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) [NBP2-46520]

Immunohistochemistry: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of Human prostate tissue.

Applications for Angiopoietin-2 Antibody (OTI3H7)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (OTI3H7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Angiopoietin-2 Antibody (OTI3H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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