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Angiopoietin-2 Antibody (S04-7E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20029

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-20029-100ul
NBP3-20029-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-7E6

Concentration

0.3 mg/ml

Product Summary for Angiopoietin-2 Antibody (S04-7E6)

Immunogen

A synthetic peptide of human Angiopoietin-2 (Uniprot # O15123)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-2 Antibody (S04-7E6)

Angiopoietin-2 Antibody (S04-7E6)

Western Blot: Angiopoietin-2 Antibody (S04-7E6) [NBP3-20029] -

Western Blot: Angiopoietin-2 Antibody (S04-7E6) [NBP3-20029] - All lanes use NBP3-20029 at 1:6000 dilution for 1 hour at room temperature.

Applications for Angiopoietin-2 Antibody (S04-7E6)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (S04-7E6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiopoietin-2 Antibody (S04-7E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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